Process for the detection of the presence of an allergy and for the specific detection of the allergen responsible for the allergy

US4592997A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Otto-Henning Wilhelms · Weinheim, DE
• Peter Stahl · New York, US
• Peter Wunderwald · Haunshofen, DE

Key dates

• Filing date: Mar 17, 1983
• Grant date: Jun 3, 1986
• Expiry date: Mar 17, 2003

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2337/12
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides a process for the detection of the presence of an allergy and for the specific detection of the allergen responsible for the allergy, based on the cellular principle, wherein leukocytes of a sample to be investigated are incubated with an allergen or another stimulation factor of degranulation, such as anti-IgE, in an aqueous medium, the leukocytes are then separated off and the remaining solution is incubated with a compound of the general formula: EQU Y--X--C in which Y is an amino acid or a peptide containing 2 or 3 amino acids, the amino acids optionally carrying a conventional protective group on the amino end, X is arginine or lysine and C is a chromogenic residue, in the presence of a buffer effective at pH 6.3 to 8.0 and the liberation of the chromogenic residue is measured.