Process for the immobilization of cyclodextrine glycosyltransferase enzyme
US4593004A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 24, 1983 |
| Grant date | Jun 3, 1986 |
| Priority date | — |
| Expiry date | Aug 24, 2003 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N11/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a process for the preparation of a cyclodextrine glycosyltransferase enzyme. The immobilization can be carried out by two methods. According to the first method a cyclodextrine glycosyltransferase enzyme is activated with a solution of a carbodiimide compound and the thus treated enzyme is applied in a solution having a pH value of 4.5-8.5 onto a polysaccharide derivative comprising at least 0.1 m-equiv./g of free amino group. According to the second method the enzyme is applied in a solution having a pH value of 4.5-8.5 onto a polymer produced from an acrylic acid and/or methacrylic acid or acrylic amide and/or methacrylic amide monomer by means of a cross-linking agent of the acrylic or allylic type which polymer comprises at least 0.1 m-equiv./g of functional carboxy group, activating the polymer with a solution of a carbodiimide compound and washing and if desired drying the product thus obtained. The advantage of the process of the present invention is that the thus immobilized enzyme--contrary to the dissolved enzyme--is suitable for longlasting application and can be readily used on industrial scale continuous production.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.