Process for the production of cell stimulating agents
US4599176A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | May 19, 1983 |
| Grant date | Jul 8, 1986 |
| Priority date | — |
| Expiry date | May 19, 2003 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01D61/145
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The invention concerns a process for obtaining cell respiratory stimulating active agents having a molecular weight in the range of about 300 to 8000 Dalton, by defibrination of calves blood, hemolyzing the solution obtained thereby, separating the proteins and substances having a molecular weight of over about 8000 Dalton comprised in the hemolyzed solution by a membrane separation procedure, concentrating the resulting protein- and high molecular weight-freed solution under reduced pressure at a temperature not exceeding 40.degree. C. to a density in the range of 1.10 to 1.15 g/ml at 20.degree. C. and partially separating the inorganic salts from the resulting concentrated solution, wherein the membrane separation procedure employed is a continuous multi-step ultrafiltration procedure employing membranes with a molecular weight exclusion limit of about over 8000 Dalton and the partial separation of the inorganic salts is carried out by electrodialysis with the aid of membranes with a membrane permeability up to a molecular weight of about 300 Dalton.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.