Method for elimination of selected cell populations in analytic cytology
US4599307A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 18, 1983 |
| Grant date | Jul 8, 1986 |
| Priority date | — |
| Expiry date | Jul 18, 2003 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/56972
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for identifying subpopulations of cells of interest without interference from other cells. In the method, a sample of at least three (3) types of cells, including a first and second type of cell which form a subpopulation of cells of interest and a third type of cell which interferes with the identification of said subpopulation, is divided into at least two (2) aliquots. A first antibody which is specific for the third type of interfering cells but not for the subpopulation, is labeled with two (2) fluorochromes, each of the fluorochromes having distinct emission spectra. The labeled first antibody is then added to a first one of the sample aliquots so as to label the third type of cells with the first antibody. A first analysis is performed by analyzing each cell to determine the fluorescence emitted and to count the third type of cells so as to distinguish the third type of cells from the subpopulation. The information obtained from the first analysis is retained for subsequent use. The above steps are repeated for the subpopulation of cells of interest and a second analysis is obtained. The retained information from the first analysis is used to remove the effect of th…
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