Reagent and process for the determination of the activity of the enzyme gamma-glutamyl transferase

US4603107A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Ulfert Deneke · Fürth, DE
• Rolf Nagel · Bürstadt, DE
• Walter Rittersdorf · Mannheim, DE

Key dates

• Filing date: Sep 16, 1983
• Grant date: Jul 29, 1986
• Expiry date: Sep 16, 2003

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/81
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides a process for the kinetic determination of .gamma.-glutamyl transferase activity by the reaction of a .gamma.-glutamylarylamide with the formation of an arylamine at a pH value of from 6 to 10, wherein the arylamine is simultaneously reacted with an aromatic amine or phenol appropriate as a coupling component and an appropriate oxidation agent to give a colored compound with an absorption maximum above 565 nm, the rate of formation of which is monitored photometrically and is used as a measure for the .gamma.-glutamyl transferase activity. The present invention also provides a reagent for the kinetic determination of .gamma.-GT activity, wherein it contains the following reagents: PA0 .gamma.-GT-arylamide:1-100 mmol/l. PA0 .gamma.-glutamyl acceptor (glycylglycine):10-500 mmol/l. PA0 coupling component:1-500 mmol/l. PA0 oxidation agent:0.1-500 mmol/l. PA0 buffer:10-1000 mmol/l.