Enzyme/immunofluorescent assay for anti-Epstein-Barr Virus antibodies
US4607008A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 5, 1984 |
| Grant date | Aug 19, 1986 |
| Priority date | — |
| Expiry date | Mar 5, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/968
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for the determination of anti-Epstein-Barr Virus antibodies in a test sample comprises contacting a substrate for the anti-Epstein-Barr Virus antibodies with sample; treating the contacted substrate with labeled antihuman Ig antibody selected from (a) a mixture comprising enzyme labeled antihuman Ig antibody and fluorescent labeled antihuman Ig antibody, (b) antihuman Ig antibodies labeled with an enzyme and a fluorescent label, (c) fluorescent labeled antihuman Ig antibody to which enzyme labeled antibody against the animal species from which the antibody used in the fluorescent labeled antibody was derived is subsequently added, and (d) enzyme labeled antihuman Ig antibody to which fluorescent labeled antibody against the animal species from which the antibody used in the enzyme labeled antibody was derived is subsequently added; determining the enzyme activity of the treated substrate; and determining the immunofluorescent patterns in substrates exhibiting enzyme activity. The method is useful for the rapid screening for anti-Epstein-Barr Virus antibodies for the diagnosis of Infectious Mononucleosis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.