Assaying for circulating immune complexes with labeled protein A
US4617262A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 22, 1983 |
| Grant date | Oct 14, 1986 |
| Priority date | — |
| Expiry date | Jul 22, 2003 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/828
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for assaying circulating immune complexes comprises PA1 contacting the circulating immune complexes in solution in serum with a staphylococcal protein-A linked to a detectable label, whereby a CIC-protein-A-label complex is formed, PA1 selectively precipitating the CIC-SPA-label complex by contacting the complex with polyethylene glycol, PA1 separating the precipitated CIC-SPA-label complex from the serum, PA1 measuring the quantity of the label in the precipitated CIC-protein-A-label complex PA1 comparing the measured quantity of label with at least one standard prepared by subjecting a solution containing a known amount of CIC or functional equivalent material to the same assay. The method requires only a single precipitation step and in a preferred embodiment the formation of the CIC-SPA-label complex may be formed in a single step.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.