Adaptor molecules for DNA and their application to synthesis of gene-derived products
US4617384A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 29, 1982 |
| Grant date | Oct 14, 1986 |
| Priority date | — |
| Expiry date | Sep 29, 2002 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S930/26
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Adaptor molecules have been prepared to comprise either start or stop signals for protein synthesis, in addition to recognition sites for restriction endonucleases. Separate adaptors may be used in a symmetrical duplex form. The start adaptor may include nucleotide base inserts to provide the correct reading frame of the triplet code in a DNA sequence with inappropriate reading frame. Insulin A-chain and B-chain genes of the human type, have been synthesized with the appropriate adaptor molecules provided on each end. The adapted DNA genes have been joined to replicable cloning vehicles and the hybrid DNA transferred to a host cell. The transformed host cell has been shown to contain the desired insulin gene.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.