Liquid chromatography/Fourier transform IR spectrometry interface flow cell
US4618769A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 4, 1985 |
| Grant date | Oct 21, 1986 |
| Priority date | — |
| Expiry date | Jan 4, 2005 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/0346
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A zero dead volume (ZDV) microbore high performance liquid chromatography (.mu.HPLC)/Fourier transform infrared (FTIR) interface flow cell includes an IR transparent crystal having a small diameter bore therein through which a sample liquid is passed. The interface flow cell further includes a metal holder in combination with a pair of inner, compressible seals for directly coupling the thus configured spectrometric flow cell to the outlet of a .mu.HPLC column end fitting to minimize the transfer volume of the effluents exiting the .mu.HPLC column which exhibit excellent flow characteristics due to the essentially unencumbered, open-flow design. The IR beam passes transverse to the sample flow through the circular bore within the IR transparent crystal, which is preferably comprised of potassium bromide (KBr) or calcium fluoride (CaF.sub.2), so as to minimize interference patterns and vignetting encountered in conventional parallel-plate IR cells. The long IR beam pathlength and lensing effect of the circular cross-section of the sample volume in combination with the refractive index differences between the solvent and the transparent crystal serve to focus the IR beam in enhancing…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.