Fluorescence intensity compensation method and device
US4622468A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 15, 1985 |
| Grant date | Nov 11, 1986 |
| Priority date | — |
| Expiry date | Jul 15, 2005 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/12753
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Variations in the intensity of a light source and the sensitivity of a fluorescence detector photo-multiplier tube are simultaneously compensated by measuring the intensity (I.sub.LS1) of the light source at the sample excitation frequency with a silicon detector, measuring the intensity (I.sub.LS2) of the light source at the emission frequency with another silicon detector, measuring with the photo-multiplier detector the intensity (I.sub.S) of the sample's fluorescence and the intensity (I.sub.LS3) of the light source at the emission frequency of the sample and then calculating a value F as representative of the sample's fluorescence according to the formula: ##EQU1##
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.