Method and apparatus for determination of an analyte and method of calibrating such apparatus
US4627014A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 9, 1984 |
| Grant date | Dec 2, 1986 |
| Priority date | — |
| Expiry date | Apr 9, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/146666
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method and apparatus are described for the determination of an analyte in an aqueous liquid while eliminating the biasing effect of unknown interferents which are preformed or formed in situ during the determination. The method comprises physically contacting a sample of the liquid with an interactive composition for the analyte; measuring the spectrophotometric responses generated by such contact at a primary wavelength .lambda..sub.1 and one or more secondary wavelengths .lambda..sub.2, .lambda..sub.3, . . . .lambda..sub.n ; and determining analyte concentration or activity using the equation: EQU C=a.sub.0 +a.sub.1 [A.sub.1 +.alpha..sub.1 A.sub.2 +. . . +.alpha..sub.n-1 A.sub.n ] wherein C is analyte concentration or activity, A.sub.1, A.sub.2, . . . A.sub.n are the spectrophotometric responses observed at .lambda..sub.1, .lambda..sub.2, . . . .lambda..sub.n, respectively; and a.sub.0, a.sub.1, and .alpha..sub.1, .alpha..sub.2, . . . .alpha..sub.n-1 are constants determined according to a calibrating method, also described herein. Such calibrating method is an empirical means for determining and recording in a chemical analyzer the a.sub.0, a.sub.1, and .alpha..sub.1, .alpha..…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.