Fibrin adhesive prepared as a concentrate from single donor fresh frozen plasma
US4627879A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 3, 1985 |
| Grant date | Dec 9, 1986 |
| Priority date | — |
| Expiry date | Jan 3, 2005 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61L24/106
- WIPO fieldMedical technology
- WIPO sectorInstruments
Abstract
This invention concerns a method of preparing a cryoprecipitated suspension containing fibrinogen and Factor XIII useful as a precursor in the preparation of a fibrin glue which involves (a) freezing fresh frozen plasma from a single donor such as a human or other animal, e.g. a cow, sheep or pig, which has been screened for blood transmitted diseases, e.g. one or more of syphilis, hepatitis or acquired immune deficiency syndrome at about =80.degree. C. for at least about 6 hours, preferably for at least about 12 hours; (b) raising the temperature of the frozen plasma, e.g. to between about 0.degree. C. and room temperature, so as to form a supernatant and a cryoprecipitated suspension containing fibrinogen and Factor XIII; and (c) recovering the cryoprecipitated suspension. The invention also concerns a method of preparing a fibrin glue useful in surgical procedures which comprises: (a) preparing a cryoprecipitated suspension as described above; (b) applying a defined volume of the suspension to a desired site; and (c) applying a composition containing a sufficient amount of thrombin, e.g. human, bovine, ovine or porcine thrombin, to the site so as to cause the fibrinogen in the s…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.