Positive selection sorting of cells
US4629687A · kind A · utility
93Cited by
17References
8Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 29, 1982 |
| Grant date | Dec 16, 1986 |
| Priority date | — |
| Expiry date | Jul 29, 2002 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N15/149
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method and apparatus for positive selection of viable cells based upon differing chemical or physical properties or dynamic processes using a focussed radiant energy beam, such as a laser beam (11) to kill unwanted cells is described. The apparatus includes a microscope (14) with a photomultiplier (27) which is used to selectively detect light from certain cells produced by an attenuated laser beam irradiating the cells for identifying the cells to be killed or saved. Based upon identification, selected cells to be killed are exposed to the unattenuated laser beam.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.