Binding assay with amplified read-out and gas-phase detection
US4629689A · kind A · utility
20Cited by
2References
30Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 29, 1984 |
| Grant date | Dec 16, 1986 |
| Priority date | — |
| Expiry date | Aug 29, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
At the conclusion of a selective binding assay (e.g., immunological or nucleic acid), an enzyme is present in modulated concentration and/or activity to moderate a chemical reaction such as the cleavage of o-nitrophenyl-.beta.-D-galactopyranoside. After a controlled period, the enzymatic product is transferred to the gas phase and concentrated relative to other components in the enzymatic reaction mixture, such as by extraction into ethyl ether, injection into a gas chromatography column and detection by flame ionization or electron capture. Kits for such assays are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.