Plasmid cloning and expression vectors for use in microorganisms
US4634678A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 13, 1982 |
| Grant date | Jan 6, 1987 |
| Priority date | — |
| Expiry date | Dec 13, 2002 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/70
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
DNA cloning and expression vectors capable of replication in a microbial host comprising from upstream to downstream (a) at least one promoter; (b) a translation start codon; (c) a cloning segment which provides a means for inserting nucleic acid sequences and (d) a sequence coding for a detectable gene product, out of translational phase with the translation start codon but capable of being readjusted to the translational phase of said start codon by insertion into the cloning segment of nucleic acid sequences containing the proper number of nucleotides for readjustment, said gene product providing a means for detecting expression of inserted nucleic acid sequences.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.