Assay processes and materials therefor
US4637985A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 27, 1983 |
| Grant date | Jan 20, 1987 |
| Priority date | — |
| Expiry date | Sep 27, 2003 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/826
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A process of carrying out a specific binding assay, for the qualitative detection or quantitative or semi-quantitative determination of an analyte which forms a component of a specific binding reaction, using a labelled conjugate form of a derivative or analogue of one of the relevant specific binding partners, said labelled form being capable of emitting delayed luminescence upon photo-excitation, characterized in that the labelled conjugate form of one of the relevant specific binding partners comprises a derivative or analogue of the specific binding partner with a derivative of a luminescent substance which shows a delayed light emission upon photoexcitation, the delayed light emission of which is subject to quenching by molecular oxygen, and characterized further in that the assay is carried out by the use of a time-resolved photometric method in the presence of an effective amount of a quench-inhibiting substance able to prevent quenching by molecular oxygen.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.