Flow cytometry lysing reagent with leukoprotective agent for producing a 3-part WBC count
US4637986A · kind A · utility
25Cited by
13References
4Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 22, 1983 |
| Grant date | Jan 20, 1987 |
| Priority date | — |
| Expiry date | Aug 22, 2003 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/108331
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Lysing reagents for use in flow cytometry instruments. Lysing reagents suitable for lysing red blood cells are provided thereby permitting 3-part differential counting of white blood cells. The reagents comprise a leukoprotective agent for preserving the lymphocyte cellular integrity during analysis, and buffers adjusted to provide the correct pH environment for optimal lysis and activity. Preferred embodiments will additionally incorporate non-ionic preservatives in very low concentrations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.