Materials for determining fructosamine levels in blood samples
US4645742A · kind A · utility
Inventor
Key dates
| Filing date | Jul 18, 1984 |
| Grant date | Feb 24, 1987 |
| Priority date | — |
| Expiry date | Jul 18, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/144444
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The determination of fructosamine levels in at least part of a blood sample for use in detecting diabetes in patients or deciding treatment levels is effected by controlling the temperature and pH of the sample, adding a coloring agent such as a nitro-blue tetrazolium and after a first delay in time taking a first color measurment at a predetermined wavelength and after a second delay in time taking a second color measurement at the predetermined wavelength, then comparing any resultant change between the first and second color measurements with those of standard solutions, the selected timing delays, wavelength, coloring agent and pH conditions being such that any change of color in the coloring agent between the first and second color measurements is caused predominantly by the glucose in the sample that is reacted with an amine group of protein and has undergone a molecular rearrangement to form fructosamine and not materially by any non-specific reducing substance which may be present in the sample. Also disclosed are the reagent and standard solutions for use in the method of detecting fructosamine levels.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.