Method for determination of hydrogen peroxide by chemiluminescence analysis
US4647532A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 22, 1985 |
| Grant date | Mar 3, 1987 |
| Priority date | — |
| Expiry date | Mar 22, 2005 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/206664
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for determination of hydrogen peroxide by chemiluminescence analysis which consists of reacting a sample containing hydrogen peroxide with an oxidizable non-fluorescent substance in the presence of an oxidizing catalyst to convert the non-fluorescent substance to a fluorescent substance, reacting the fluorescent substance with an oxalic acid di-ester and hydrogen peroxide in the presence of an inhibitor which inactivates the oxidizing catalyst, and then measuring the amount of light emission produced to determine the amount of hydrogen peroxide contained in the sample. Determination and enzyme immunoassay for a component other than hydrogen peroxide in a sample from living body and evaluation of bactericidal activity in phagocytosis of leukocytes by utilizing this method are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.