Patent · US Expired

Method for the isolation and purification of hepatitis B surface antigen using polysorbate

US4649192A · kind A · utility

20Cited by

10References

9Claims

0Family size

Assignee

Smith Kline - RIT · BE

Inventors

Frans Van Wijnendaele · Ottenburg, BE
Guy Simonet · Paris, FR

Key dates

Filing date	May 30, 1985
Grant date	Mar 10, 1987
Priority date	—
Expiry date	May 30, 2005

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY10S530/826
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The supernatant of engineered yeast cells having produced hepatitis B surface antigaen and disrupted in the presence of a polysorbate non-ionic detergent is clarified by addition of urea and adsorbed on colloidal silica from which the purified antigen is desorbed with a low ionic strength buffer supplemented with urea and a polysorbate non-ionic detergent.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.