Isolation of carboxypeptidase B enzyme
US4650762A · kind A · utility
3Cited by
5References
8Claims
0Family size
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Key dates
| Filing date | Apr 2, 1984 |
| Grant date | Mar 17, 1987 |
| Priority date | — |
| Expiry date | Apr 2, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/816
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Carboxypeptidase B enzyme is isolated by a process wherein homogenized and autolyzed pancreas is subjected to a thermal treatment, and carboxypeptidase B is separated by fractionation with ammonium sulfate. This process produces carboxypeptidase B having satisfactory specific activity while eliminating the use of pancreas cell fluid or an acetonous powder from pancreas as a starting material. The isolated carboxypeptidase B may be immobilized on a polymer gel containing carboxy groups activated with a carbodiimide.
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