Methods for the in vitro detection and identification of unknown pathogens or genetic entities
US4652517A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 11, 1984 |
| Grant date | Mar 24, 1987 |
| Priority date | — |
| Expiry date | Jun 11, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/803
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for the detection and identification of unknown pathogens or genetic entities from samples suspected of containing the same involve the use of novel wicking techniques whereby sample DNA/RNA becomes readily available on a porous, inert, positively charged support in single stranded form for hybridization with a hybridization probe having a nucleotide sequence substantially complementary to a nucleotide sequence of the sample DNA/RNA. Such methods permit the detection and identification of unknown pathogens and genetic entities in a shorter period of time than that required by conventional methods. Lysis solutions for rapidly lysing bacterial cells without the aid of enzymes are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.