Manufacture and expression of structural genes
US4652639A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | May 6, 1982 |
| Grant date | Mar 24, 1987 |
| Priority date | — |
| Expiry date | May 6, 2002 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/60
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Described are rapid and highly efficient procedures for the total synthesis of linear, double stranded DNA sequences of up to about 200 base pairs, which sequences may comprise entire structural genes. Illustratively disclosed is the preparation and expression of manufactured genes, including fusion genes, capable of directing synthesis of human .beta.-endorphin and of proteins which differ from human .beta.-endorphin in terms of the identity or relative position of one or more amino acids. Manufactured genes preferably include codons selected from among alternative codons specifying the same amino acid on the basis of preferential expression in a projected host microorganism (e.g., E. coli) to be transformed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.