Separation and purification of biomembrane proteins
US4654132A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 24, 1985 |
| Grant date | Mar 31, 1987 |
| Priority date | — |
| Expiry date | Jul 24, 2005 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44747
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for separating and purifying a biomembrane protein from biomembrane by subjecting the biomembrane to gel electrophoresis in the presence of at least one anionic surfactant having the general formula (I): EQU RO--XO).sub.m (YO).sub.n SO.sub.3 M (I) wherein R represents an alkyl group having 6 to 22 carbon atoms or an alkylphenyl group having 6 to 22 carbon atoms, X and Y independently represent a hydrocarbon residue having 1 to 4 carbon atoms, m and n independently represent a number of from zero to 40 provided that m+n is 4 to 40, and M represents an alkali metal, an alkaline earth metal, an amine, or ammonium. Thus, the desired biomembrane protein can be separated and purified with a high purity without denaturing the protein and also without impairing the biological function thereof.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.