Fluorescent microbead quenching assay
US4654300A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 2, 1982 |
| Grant date | Mar 31, 1987 |
| Priority date | — |
| Expiry date | Apr 2, 2002 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/966
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Method and compositions for performing immunoassays having conjugated fluorescent particles and conjugated catalyst where the particles and catalysts are conjugated to members of a specific binding pair. Bound to the fluorescent particle is a catalyst, usually enzyme, member of a signal producing system, which system includes the fluorescent particle. Another catalyst is bound to a specific binding pair member. The catalyst-specific binding member conjugate becomes bound to the particle, by the intermediacy of the binding of the specific binding pair, producing a quenching product which binds to the particle, resulting in a reduction in fluorescence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.