Gene amplification in eukaryotic cells
US4656134A · kind A · utility
141Cited by
0References
9Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Apr 12, 1985 |
| Grant date | Apr 7, 1987 |
| Priority date | — |
| Expiry date | Apr 12, 2005 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/85
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Method and compositions are provided for gene amplification. A DNA unit is prepared capable of replication in a eukaryotic host having tandem genes, where the first gene is capable of complementing an auxotrophic host. The auxotrophic host is transformed with the DNA unit under selective conditions requiring enhanced expression of the first gene. Hosts are then selected for amplification of the first and second genes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.