Yeast of the genus kluyveromyces modified for the expression of preprothaumatin or its various allelic and modified forms or their maturation forms, and the proteins obtained by that expression
US4657857A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 18, 1984 |
| Grant date | Apr 14, 1987 |
| Priority date | — |
| Expiry date | Jan 18, 2004 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/036
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to yeasts of the genus Kluyveromyces, in particular K. lactis, acting as hosts for the expression of preprothaumatin or its various allelic and modified forms or their maturation forms, which yeasts contain an rDNA plasmid comprising a structural gene encoding for a thaumatin-like protein, an autonomous replicating sequence derived from K. lactis (KARS), a yeast regulon derived from the GAPDH-genes of S. cerevisiae, and a selection marker, e.g. the trp-1 or the lac-4 gene from S. cerevisiae and K. lactis, respectively. Optionally the plasmid also contains a transcription terminator from a yeast. Further the preparation of such yeasts and the preparation of thaumatin-like proteins with such yeasts and the proteins so obtained are claimed. The yeast described give a better expression than obtained with E. coli as described in EP-PA (A2) 0 054 330 and 0 054 331, published on June 23, 1982.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.