Process for the treatment of fermentation broth containing vitamin B.sub.12 and other corrinoids and for the preparation of vitamin B.sub.12 concentrates
US4657859A · kind A · utility
Inventors
Key dates
| Filing date | Feb 6, 1984 |
| Grant date | Apr 14, 1987 |
| Priority date | — |
| Expiry date | Feb 6, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/803
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process is disclosed for the treatment of fermentation broths containing vitamin B.sub.12 and other corrinoids, and for the preparation of vitamin B.sub.12 concentrates or crystalline vitamin B.sub.12 wherein the fermentation broth is contacted with a nonionic, macroreticular, adsorption resin having a pore size of 10.sup.-8 to 10.sup.-7 meters, a grain diameter of at least 10.sup.-4 m, and a specific surface area of at least 200 m.sup.2 /g so that the macromolecular adsorption resin adsorbs fermentation broth additives and metabolites of fermentation, whereas the intact microorganism cells containing the vitamin B.sub.12 remain in solution. The solution of intact vitamin B.sub.12 -containing cells is then treated further to obtain vitamin B.sub.12 in still purer form.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.