Stabilization of a mutant microorganism population
US4657863A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 11, 1983 |
| Grant date | Apr 14, 1987 |
| Priority date | — |
| Expiry date | Apr 11, 2003 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P1/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention provides a process for stabilizing a population of a mutant microorganism in a bioconversion system whereby growth of revertant cells is suppressed. The process involves limiting a nutrient which is essential for cell growth so that the cells selectively grow on a growth carbon source rather than on a non-growth carbon source which is present. The revertant cells being suppressed have a similar ability as the parent strain of the mutant microorganism to grow on the non-growth carbon source in the bioconversion system. The non-growth carbon source in the bioconversion system is metabolized to an extracellular accumulating quantity of a desired metabolite, e.g., toluene is converted to muconic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.