Pure alkaline phosphatase, its preparation and use
US4659666A · kind A · utility
17Cited by
1References
4Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 26, 1984 |
| Grant date | Apr 21, 1987 |
| Priority date | — |
| Expiry date | Oct 26, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/815
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The enzyme alkaline phosphatase having the following properties: PAC (i) PA0 molecular weight: approximately 80 000 with 2 subunits of 40 000 PAC (iii) PA0 activator: Mg.sup.++ PA0 inhibitor: EDTA PAC (iii) PA0 thermal stability: 45.degree. C. or below PAC (iv) PA0 optimum temperature: 35.degree.-45.degree. C. PAC (v) PA0 pH stability: 6-11 PAC (vi) PA0 pH optimum: 10 PAC (vii) PA0 specific activity: 3000-5000 units/mg PAC (viii) PA0 isoelectric range: between pH 5.0 and 6.0. An antibody-enzyme conjugate process for purifying alkaline phosphatase and a reagent test kit using the purified alkaline phosphatase are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.