Method of sequencing peptides
US4665037A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Apr 28, 1986 |
| Grant date | May 12, 1987 |
| Priority date | — |
| Expiry date | Apr 28, 2006 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/255
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of sequencing polypeptides is disclosed utilizing liquid-solid affinity chromotography. The method utilizes a reagent reactive at one position with the amino moiety of a terminal amino acid, and reactive at a second position with boronic acid. The reagent is coupled to the terminal amino acid. A scavenger molecule having a sulfhydryl group and an amine group reacts with the excess reagent and the excess scavenger and scavenger-reagent complex are removed on an immobilized organomercurial column. The terminal amino acid is cleaved from the polypeptide, and coupled to an immobilized boronic acid column. The amino acid is removed from the column and identified and the remainder polypeptide is recycled.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.