Immunoassay with chromatographic medium and labelled reagent
US4666863A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 22, 1984 |
| Grant date | May 19, 1987 |
| Priority date | — |
| Expiry date | Feb 22, 2004 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/74
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of performing an assay for an analyte comprises providing a chromatographic medium, preferably in sheet form, forming a reaction mixture containing a labelled reagent present partly in a form which is mobile on the medium and partly in a form which is immobile, the proportions of the two forms being related to the analyte concentration, applying the mixture to a spot on the chromatographic medium, optionally applying a solvent to cause the mobile form of the labelled reagent to migrate from the spot, and measuring the label intensity remaining at the spot. The method provides a convenient way of performing immunoassays, 2-site immunometric assays, and agglutination assays.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.