Reducing background interference activity in enzyme-label immunoassays
US4668620A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 22, 1984 |
| Grant date | May 26, 1987 |
| Priority date | — |
| Expiry date | Feb 22, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/825
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Background interference which disrupts the measurement of an analyte in enzyme immunoassays with samples is substantially diminished. In accordance with one embodiment of the invention, the serum sample is treated with a sufficient amount of a peracid compound, either organic or inorganic, for a time and under conditions sufficient to reduce or eliminate background interference contributed by serum components other than analyte. The peracid compound is readily quenched, without adversely affecting the assay compositions. In another embodiment of the invention a liqand for the interfering component of the sample, such as unconjugated enzyme which is inactive but antigenic, is mixed with the sample to be analyzed. The presence of the liqand substantially reduces or eliminates background interference from components of the sample other than analyte.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.