Heterogeneous immunoassay utilizing horizontal separation in an analytical element
US4670381A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 19, 1985 |
| Grant date | Jun 2, 1987 |
| Priority date | — |
| Expiry date | Jul 19, 2005 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/816
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A heterogeneous, competitive binding immunoassay is conducted with a dry analytical element. The immunoassay is useful for determining a ligand (e.g. a therapeutic drug) and includes contacting a finite area of the element spreading layer with a sample of a liquid in the presence of a labeled ligand analog and an immobilized receptor. An immobilized ligand-receptor complex is formed within the finite area. Simultaneously, the uncomplexed ligand migrates horizontally away from the immobilized complex which remains in the center of the finite area. At least five seconds after the completion of the contacting, the amount of immobilized complex is measured in the center of the finite area.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.