Controlled regeneration of cotton plants from tissue culture
US4672035A · kind A · utility
40Cited by
3References
13Claims
0Family size
Assignee
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Key dates
| Filing date | Mar 16, 1984 |
| Grant date | Jun 9, 1987 |
| Priority date | — |
| Expiry date | Mar 16, 2004 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01H4/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The regeneration of plants from cotton callus (Gossypium hirsutum L. cv Coker 310) is described. Somatic proembryoids developed spontaneously after two years in culture on a modified Linsmaier and Skoog medium. The percentage of calli forming proembryoids was increased to about 30% by prolonged culture without NAA and kinetin. Development of proembryoids was enhanced by transferring proembryoids to media lacking NH.sub.4 NO.sub.3 but containing double the standard KNO.sub.3 concentration and gibberellic acid. Root initiation and growth was promoted by lowering the glucose concentration to 5 g/l.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.