Method of measuring the number of eumycete cells
US4675289A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 12, 1984 |
| Grant date | Jun 23, 1987 |
| Priority date | — |
| Expiry date | Apr 12, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/911
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of measuring the number of eumycete cells in a sample which comprises preparing a solution or suspension containing a sample of a medicine, food, drink, cosmetic or water, adding to said solution or suspension a 7-amino-4-methyl-coumarin derivative represented by formula (1): ##STR1## wherein R is an alkyl group, an allyl group, an aralkyl group, or a heterocyclic group, or R--CO-- is an amino acid or peptide residue, said derivative not inhibiting the hydrolysis of the amide bond of formula (1) by microorganism hydrolases contained in the samples; and measuring the fluorescence of 7-amino-4-methyl-courmarin released by the microorganism hydrolases.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.