Hybrid protein
US4675382A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Nov 6, 1985 |
| Grant date | Jun 23, 1987 |
| Priority date | — |
| Expiry date | Nov 6, 2005 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S930/31
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A hybrid protein including protein fragments joined together by peptide bonds, the hybrid protein including, in sequential order, beginning at the amino terminal end of the hybrid protein, PA1 (a) the enzymatically active Fragment A of diphtheria PA1 (b) a fragment including the cleavage domain 1.sub.1 adjacent the Fragment A of diphtheria toxin, PA1 (c) a fragment including at least a portion of the hydrophobic domain of Fragment B of diphtheria toxin and not including the generalized eukaryotic binding site of the Fragment B, and PA1 (d) a fragment including a portion of a cell-specific polypeptide ligand, the portion including at least a portion of the binding domain of the polypeptide ligand, the portion of the binding domain being effective to cause the hybrid protein to bind selectively to a predetermined class of cells to be attacked by the enzymatically active Fragment A.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.