Purification of T-cell growth factor
US4675383A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 9, 1984 |
| Grant date | Jun 23, 1987 |
| Priority date | — |
| Expiry date | Nov 9, 2004 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/55
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Substantially homogenous T-cell growth factor is isolated. A multiple-step purification procedure of T-cell growth factor-containing biological sample includes at least one reverse-phase HPLC fractionation using a C3 to C5 silica gel stationary phase. A preferred pre-purification protocol includes concentration of the biological sample on a semipermeable medium followed by gel filtration and semi-preparative reverse-phase HPLC on a hydrophobic stationary phase. Fractionating the pre-purified biological sample with two or three reverse-phase HPLC fractionations using C3 to C5 silica gel stationary phase yields T-cells growth factor of over 90% purity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.