Human tumor necrosis factor

US4677063A · kind A · utility

Assignee

• Cetus Corporation · US

Inventors

• David F. Mark · Plainsboro, US
• Alice M. Wang · Lafayette, US
• Martha B. Ladner · Oakland, US
• Abla A. Creasey · Piedmont, US
• Leo S. Lin · Walnut Creek, US
• Janelle N. Van Arsdell · Oakland, US

Key dates

• Filing date: Jul 30, 1985
• Grant date: Jun 30, 1987
• Expiry date: Jul 30, 2005

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S930/144
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Human tumor necrosis factor (TNF) has been prepared using recombinant methods. A human promyelocytic leukemia cell line has been induced using an improved induction procedure, and the TNF purified to homogeneity. Methods, vectors, and cells useful in obtaining human TNF in practical amounts are disclosed. Muteins having N-terminal deletions, which muteins have superior biological activity, are also disclosed.