Human tumor necrosis factor

US4677064A · kind A · utility

Assignee

• Cetus Corporation · US

Inventors

• David F. Mark · Plainsboro, US
• Alice M. Wang · Lafayette, US
• Martha B. Ladner · Oakland, US
• Abla A. Creasey · Piedmont, US
• Janelle N. Van Arsdell · Oakland, US
• Leo S. Lin · Walnut Creek, US

Key dates

• Filing date: May 2, 1985
• Grant date: Jun 30, 1987
• Expiry date: May 2, 2005

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S930/144
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Human Tumor necrosis factor (TNF) has been prepared using recombinant methods. A human promyelocytic leukemia cell line has been induced using an improved induction procedure, and the TNF purified to homogeneity. Methods, vectors, and cells useful in obtaining human TNF in practical amounts are disclosed. Muteins having N-terminal deletions, which muteins have superior biological activity, are also disclosed.