Purification and activation of proteins from insoluble inclusion bodies
US4677196A · kind A · utility
45Cited by
4References
20Claims
0Family size
Assignee
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Key dates
| Filing date | Sep 6, 1985 |
| Grant date | Jun 30, 1987 |
| Priority date | — |
| Expiry date | Sep 6, 2005 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/82
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Purification and activation of proteins produced in transformant microorganisms as insoluble, biologically inactive inclusion bodies can be effected by solubilizing the inclusion bodies in SDS; dialyzing or refrigerating the protein solution to remove a portion of the SDS; chromatographing the protein solution on an ion-retardation resin to remove the remaining SDS from the protein; and chromatographing the protein solution thus obtained on an anion-exchange resin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.