Renaturing reversibly denatured polypeptides and proteins by electrodialysis of solutions thereof in denaturants
US4678553A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 11, 1986 |
| Grant date | Jul 7, 1987 |
| Priority date | — |
| Expiry date | Jul 11, 2006 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K1/1136
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A process is disclosed for recovering renatured polypeptides and proteins (collectively "polypeptides") from solution thereof and/or of their S-sulfonate derivatives (collectively "solution of polypeptides") in denaturants comprising: PA0 introducing said solution into a renaturant chamber (compartment, cell, space) of an electrodialysis ("ED") apparatus, said chamber bounded by membranes each of which is substantially permeable to low molecular weight singly charged ions of at least one charge sign and substantially impermeable to said polypeptides and S-sulfonate derivatives, said membranes separating said renaturant chamber from juxtaposed rinsing chambers on each side of the renaturant chamber; PA0 introducing rinsing solutions into said juxtaposed rinsing chambers, said rinsing solutions synergetically with said membranes facilitating a decrease in the chaotropism of said denaturant in said renaturant chamber when a direct electric current is imposed through said renaturant and rinsing chambers in series; PA0 applying a direct electric current (which may have a substantial alternating current component) in series through said juxtaposed renaturant and rinsing chambers in a dir…
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