Analytical element and method for colorimetric determination of total cholesterol
US4680259A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 26, 1984 |
| Grant date | Jul 14, 1987 |
| Priority date | — |
| Expiry date | Sep 26, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/805
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An analytical element is designed for the colorimetric determination of total cholesterol in aqueous liquids, such as biological fluids. This element comprises an absorbent material and critical amounts of cholesterol ester hydrolase (from about 1500 to about 12,000 I.U./m.sup.2) and a nonionic surfactant (from about 5 and up to, but less than 11 g/m.sup.2). The use of these ranges of reagents reduces the potential for interference by triglycerides present in a test sample. Total cholesterol is determined with this element by detection of a color change resulting from a series of enzymatic reactions which produce hydrogen peroxide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.