Major iron-regulated protein of Neisseria gonorrhoeae and its use as vaccine

US4681761A · kind A · utility

Assignee

• State of Oregon, acting by and through the Oregon State Board of Higer Education, acting for and on behalf of the Oregon Health Sciences University · US

Inventors

• Timothy A. Mietzner · Pittsburgh, US
• Stephen A. Morse · Menlo Park, US

Key dates

• Filing date: Oct 24, 1985
• Grant date: Jul 21, 1987
• Expiry date: Oct 24, 2005

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/825
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The major iron-regulated protein of a pathogenic species of the genus Neisseria, the gonococcal congener of which (MIRP) protein has a molecular weight of approximately 37,000 daltons and consisting of about 340 amino acids, is isolated in an immunospecific antigenically substantially pure form suitable for use as a vaccine against the pathogenic species of the genus Neisseria effective to stimulate the production of a protective level of antibodies to the pathogenic species in a susceptible host, by the steps of: PA0 growing cells of that pathogenic species in an iron-depleted bacteriologic media; PA0 harvesting the thus-grown cells; PA0 disrupting the cells; PA0 separating the soluble portion of the cells from the insoluble portion; and PA0 selectivity solubilizing the MIRP from the insoluble portion of the disrupted cells with an aqueous cationic surfactant aqueous media, e.g., cetyltrimethylammonium bromide; and PA0 removing residual contaminates in the MIRP fraction by chromatographic fractionation.