Method for detection of polymorphic restriction sites and nucleic acid sequences
US4683194A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 28, 1985 |
| Grant date | Jul 28, 1987 |
| Priority date | — |
| Expiry date | Mar 28, 2005 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/811
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
In a method for detecting the presence or absence of a specific restriction site in a nucleic acid sequence an oligonucleotide probe complementary to one strand of the nucleic acid sequence spanning said restriction site is synthesized. The probe is labeled at the end nearer the restriction site. The nucleic acid is hybridized to the probe and a blocking oligomer may be added, if necessary, to prevent non-specific binding of the probe. Subsequent digestion with a restriction enzyme cleaves those oligomers that have hybridized to the nucleic acid and reformed the restriction site. The resulting cut and uncut labeled oligomers are separated and detected based on the type of probe label. The described method may be used to detect sickle cell anemia.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.