Mammalian suppressor genes
US4687737A · kind A · utility
10Cited by
0References
21Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 2, 1985 |
| Grant date | Aug 18, 1987 |
| Priority date | — |
| Expiry date | Jul 2, 2005 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of suppressing a nonsense codon in a gene in a mammalian cell by preparing an oligonucleotide primer having a mismatched anticodon region corresponding to the nonsense codon; preparing a DNA template for production of a tRNA molecule enabling the insertion of an amino acid when the nonsense codon is translated; forming a suppressor gene from said template and primer by site specific mutagenesis; and transforming the suppressor gene into the cell.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.