Composition and method for rapid detection of microorganisms in clinical samples
US4693972A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 16, 1984 |
| Grant date | Sep 15, 1987 |
| Priority date | — |
| Expiry date | Jan 16, 2004 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2304/12
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for rapid detection of microorganisms in a body fluid sample includes detecting the microorganisms after treatment of the sample with a lysing agent in order to dissolve sample components other than microorganisms, and staining with a fluorescent dye. The lysing agent may be part of a composition which includes a culture medium thereby providing simultaneous lysis and growth before staining. Detection is preferably accomplished by reliance on the fluorescence emitted by the dye having been properly excited by light energy. A composition suitable for use in the above-described method includes a growth medium and a lysing agent.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.