cDNA clones coding for polypeptides exhibiting multi-lineage cellular growth factor activity
US4695542A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 19, 1984 |
| Grant date | Sep 22, 1987 |
| Priority date | — |
| Expiry date | Mar 19, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S930/30
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Plasmid vectors are provided that carry complementary DNA (cDNA) clones coding for polypeptides exhibiting mammalian multi-lineage growth cell activity. One of these polypeptides is 166 amino acids in length, including a potential leader sequence of about 19 amino acids. The cDNA is derived from messenger RNA isolated from a mouse T-cell line after activation with concanavalin A. The cDNA was cloned by incorporation into a plasmid vector, which was then transformed into E. coli. The plasmid vector also contains DNA segments from the SV40 virus, permitting expression of the cDNA after transfection into a mammalian host cell, such as monkey COS-7 cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.