Method for determining lipid bound sialic acid in whole blood
US4701418A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Mar 18, 1985 |
| Grant date | Oct 20, 1987 |
| Priority date | — |
| Expiry date | Mar 18, 2005 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/255
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The amount of lipid bound sialic acid in a whole blood sample may be determined by a method, which may be automated, involving the following steps: adding to the sample a lower alkyl alcohol and deionized distilled water; mixing the resulting admixture; adding to it a mixture of lower alkyl chlorinated hydrocarbon and a lower alkyl alcohol; treating by mixing and centrifuging the mixture until a recoverable, substantially clear upper phase forms; recovering the upper phase and adding to it a protein-precipitating agent, preferably in admixture with an adsorbing material; mixing the resulting admixture; recovering the resulting precipitate, suspending the precipitate in distilled water and determining the amount of lipid bound sialic acid present. The presently preferred mixture for addition to the upper phase is a mixture of 75% by weight phosphotungstic acid and 25% by weight silica gel on a dry weight basis. By so determining the amount of lipid bound sialic acid present in a whole blood sample and comparing the amount with values obtained for subjects known to have cancer one may diagnose the presence of cancer in a subject. Alternatively, by determining the amount of lipid boun…
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