Isolation of bioactive, monomeric growth hormone
US4705848A · kind A · utility
17Cited by
4References
19Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 2, 1986 |
| Grant date | Nov 10, 1987 |
| Priority date | — |
| Expiry date | Jun 2, 2006 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/825
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Monomeric, biologically active growth hormone is isolated from microbially-produced insoluble inclusion bodies by solubilizing and denaturing the growth hormone by extraction of the inclusion bodies into a guanidine salt solution such as guanidine hydrochloride and subsequently renaturing at least a portion of the growth hormone in the solution by replacing the guanidine salt solution with a denaturant-free buffer solution and removing precipitated impurities and growth hormone aggregates. The renatured growth hromone is then purified by ion-exchange chromatography.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.